1FY3

[G175Q]HBP, A mutant of human heparin binding protein (CAP37)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-08-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	38.003, 65.138, 100.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.890
R-factor	0.182 *
Rwork	0.182
R-free	0.25200
RMSD bond length	0.009
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.920
High resolution limit [Å]	1.890	1.890
Rmerge	0.063	0.269
Total number of observations	102404 *
Number of reflections	18582
<I/σ(I)>	14.7
Completeness [%]	89.3	87.7
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	Ethanol, glycerol, TRIS, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	reservoir	ethanol	2-6 (%)
3	1	reservoir	glycerol	15 (%)
4	1	reservoir	Tris	0.1 (M)