1FY3
[G175Q]HBP, A mutant of human heparin binding protein (CAP37)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.003, 65.138, 100.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.890 |
| R-factor | 0.182 * |
| Rwork | 0.182 |
| R-free | 0.25200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.063 | 0.269 |
| Total number of observations | 102404 * | |
| Number of reflections | 18582 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 89.3 | 87.7 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | Ethanol, glycerol, TRIS, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | reservoir | ethanol | 2-6 (%) | |
| 3 | 1 | reservoir | glycerol | 15 (%) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) |
