1FXZ

CRYSTAL STRUCTURE OF SOYBEAN PROGLYCININ A1AB1B HOMOTRIMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	68
Detector technology	AREA DETECTOR
Collection date	1997-01-17
Detector	SIEMENS HI-STAR
Spacegroup name	P 41
Unit cell lengths	115.524, 115.524, 146.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.199 *
Rwork	0.199
R-free	0.25000
RMSD bond length	0.013 *
RMSD bond angle	3.270 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.800
Rmerge	0.089 *
Total number of observations	142631 *
Number of reflections	46010 *
Completeness [%]	99.5
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	281 *	pH 7.6, Dialysis, temperature 302.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	8 (%)
3	1	reservoir	MES	0.1 (M)
4	1	reservoir		0.4 (M)