1FXL
CRYSTAL STRUCTURE OF HUD AND AU-RICH ELEMENT OF THE C-FOS RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-06 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.310, 99.380, 33.340 |
| Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
| Resolution | 30.450 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.23900 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.190 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.450 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.329 |
| Number of reflections | 16220 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 92.3 | 85.1 |
| Redundancy | 4.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 20 * | PEG 3350, Ammonium sulfate, Calcium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 20 * | PEG 3350, Ammonium sulfate, Calcium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | (NH4)2SO4 | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | Tris |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 30 (%(w/v)) | |
| 2 | 1 | reservoir | Tris | 50 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | 10 (mM) |
