1FXK

CRYSTAL STRUCTURE OF ARCHAEAL PREFOLDIN (GIMC).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	72.850, 90.690, 78.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 2.300
R-factor	0.218
Rwork	0.218
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	18.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.044 *	0.188 *
<I/σ(I)>	24.5	6.5
Completeness [%]	99.6	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		21 *	temperature was shifted to 4 degrees after one week of incubation *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium acetate	800 (mM)
3	1	reservoir	ethylene glycol	20 (%)