1FXJ

CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINE 1-PHOSPHATE URIDYLTRANSFERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	1999-02-16
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	142.680, 142.680, 248.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.250
Rwork	0.234
R-free	0.27400
RMSD bond length	0.013
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.250
Rmerge	0.039	0.310 *
Number of reflections	44
<I/σ(I)>	14
Completeness [%]	97.0	76.8 *
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	20 *	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	ammonium sulfate	1.3-1.5 (M)
3	1	reservoir	MES	0.1 (M)
4	1	reservoir	acetone	4 (%(v/v))