1FX8

CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-01
Detector	ADSC QUANTUM
Spacegroup name	I 4 2 2
Unit cell lengths	96.760, 96.760, 184.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.197
Rwork	0.197
R-free	0.22300
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200	2.200
Rmerge	0.074	0.393
Total number of observations	139069 *
Number of reflections	19141
<I/σ(I)>	15.8
Completeness [%]	90.9	57.7
Redundancy	7.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.9	298	GLPF at 15-20 mg/ml, 28%(w/v) PEG2K, 100mM Bicine, 15% (v/v) glycerol, 35mM n-octyl-beta-D-glucoside, 300mM MgCl2, 5mM DTT, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-20 (mg/ml)
2	1	2	PEG2000	28 (%(w/v))
3	1	2	bicine	100 (mM)
4	1	2	glycerol	15 (%(v/v))
5	1	2	n-octyl-beta-D-glucoside	35 (mM)
6	1	2		300 (mM)
7	1	2	dithiothreitol	5 (mM)