1FWX
CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-17 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 102.595, 105.090, 116.696 |
Unit cell angles | 90.00, 110.69, 90.00 |
Refinement procedure
Resolution | 29.830 - 1.600 |
R-factor | 0.241 * |
Rwork | 0.241 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qni |
RMSD bond length | 0.007 |
RMSD bond angle | 2.300 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.630 |
Rmerge | 0.051 | 0.252 |
Number of reflections | 272421 | |
<I/σ(I)> | 27.9 | |
Completeness [%] | 91.8 | 84.7 |
Redundancy | 2.05 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 4 * | Haltia, T., (2003) BIOCHEM.J., 369, 77. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
2 | 1 | reservoir | sodium cacodylate | 80 (mM) | |
3 | 1 | reservoir | magnesium acetate | 160 (mM) | |
4 | 1 | drop | protein | 14 (mg/ml) |