1FWL
CRYSTAL STRUCTURE OF HOMOSERINE KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.993, 128.721, 109.423 |
| Unit cell angles | 90.00, 105.57, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| Rwork | 0.204 |
| R-free | 0.24800 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.041 | 0.309 |
| Total number of observations | 258313 * | |
| Number of reflections | 78267 | |
| <I/σ(I)> | 29.2 | |
| Completeness [%] | 99.8 | 96.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 20 * | PEG4000, sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 10 | 1 | reservoir | Na acetate | 0.2 (M) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 6 | 1 | drop | Na acetate | 0.2 (M) | |
| 7 | 1 | drop | PEG4000 | 25 (%) | |
| 8 | 1 | reservoir | PEG4000 | 20 (%) | |
| 9 | 1 | reservoir | Tris-HCl | 0.1 (M) |
