1FWH
KLEBSIELLA AEROGENES UREASE, C319Y VARIANT
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | I 21 3 |
Unit cell lengths | 170.800, 170.800, 170.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.179 |
Rwork | 0.179 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.466 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.000 * | |
Rmerge | 0.064 | 0.306 * |
Number of reflections | 49732 | |
Completeness [%] | 91.0 | 59 * |
Redundancy | 2.3 | 1.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 25 * | Jabri, E., (1992) J.Mol.Biol., 227, 934. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | urease | 5 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | |
3 | 1 | drop | EDTA | 0.5 (mM) | |
4 | 1 | drop | beta-mercaptoethanol | 0.5 (mM) | |
5 | 1 | drop | 0.75-0.85 (M) | ||
6 | 1 | drop | HEPES | 50 (mM) | |
7 | 1 | reservoir | 1.5-1.7 (M) | ||
8 | 1 | reservoir | HEPES | 100 (mM) |