1FW1
Glutathione transferase zeta/maleylacetoacetate isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 100.000, 100.000, 56.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.760 - 1.900 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28100 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.286 |
| Total number of observations | 152479 * | |
| Number of reflections | 22750 * | |
| <I/σ(I)> | 35.3 | |
| Completeness [%] | 98.1 | 88.7 |
| Redundancy | 6.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | ammonium sulfate, tert-butanol, bicine, glutathione, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 20 (mM) | |
| 2 | 1 | drop | dithiothreitol | 2 (mM) | |
| 3 | 1 | drop | GSH | 2 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | Bicine | 100 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 7 | 1 | reservoir | GSH | 10 (mM) | |
| 8 | 1 | reservoir | ammonium sulfate | 48-52 (%sat) | |
| 9 | 1 | reservoir | tert-butyl alcohol | 1 (%) |
