1FW0
CRYSTAL STRUCTURE OF THE GLUR2 LIGAND BINDING CORE (S1S2J) IN COMPLEX WITH KAINATE AT 2.0 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.279, 89.014, 48.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.245 * |
| Rwork | 0.245 |
| R-free | 0.30800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.033 | 0.137 |
| Total number of observations | 96421 * | |
| Number of reflections | 18725 | |
| <I/σ(I)> | 29.4 | |
| Completeness [%] | 92.0 | 61.9 |
| Redundancy | 5.15 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | 10% PEG 1450, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 274K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | kainate | 10 (mM) | |
| 5 | 1 | reservoir | PEG1450 | 8-15 (%) | |
| 6 | 1 | reservoir | 0.1 (M) |
