1FVT

THE STRUCTURE OF CYCLIN-DEPENDENT KINASE 2 (CDK2) IN COMPLEX WITH AN OXINDOLE INHIBITOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1997-04-11
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	54.142, 72.711, 72.933
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.195
Rwork	0.232
R-free	0.23200
RMSD bond length	0.009
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.071	0.217
Total number of observations	157652 *
Number of reflections	13325
<I/σ(I)>	41.2
Completeness [%]	87.9	87
Redundancy	11	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	20 *	Shewchuk L., (2000) J. Med. Chem., 43, 133. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	drop	EDTA	1 (mM)
4	1	reservoir	PEG3340	10 (%)