1FVP
FLAVOPROTEIN 390
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Detector | WEISSENBERG |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 76.800, 76.800, 242.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.700 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.033 * |
| Data reduction software | WEIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.062 |
| Number of reflections | 17448 |
| Completeness [%] | 93.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 9 * | 20 * | Kita, A., (1991) J.Biochem., 110, 748. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 200-250 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 1.6-2.2 (M) | |
| 3 | 1 | reservoir | potassium phosphate | 1.8-2.5 (M) |
