1FVJ
THE 2.06 ANGSTROM STRUCTURE OF THE H32Y MUTANT OF THE DISULFIDE BOND FORMATION PROTEIN (DSBA)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-11-05 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.700, 65.100, 76.400 |
| Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.060 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.21800 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1dsb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.980 * |
| Data reduction software | R-AXIS (SOFTWARE) |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.056 | 0.219 |
| Total number of observations | 74395 * | |
| Number of reflections | 26334 | |
| <I/σ(I)> | 17.3 | 3.89 |
| Completeness [%] | 91.0 | 84.1 |
| Redundancy | 2.8 | 1.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 21 * | Martin, J.L., (1993) J.Mol.Biol., 230, 1097. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20-25 (%(w/v)) | |
| 2 | 1 | reservoir | MPD | 1 (%) | |
| 3 | 1 | reservoir | cacodylate | 0.1 (M) | |
| 4 | 1 | drop | MPD | 1 (%) | |
| 5 | 1 | drop | PEG8000 | 20-25 (%(w/v)) | |
| 6 | 1 | drop | cacodylate | 0.1 (M) | |
| 7 | 1 | drop | DsbA solution | 0.002ml |
