1FV3
THE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH AN ANALOGUE OF ITS GANGLIOSIDE RECEPTOR GT1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.311, 53.068, 118.278 |
| Unit cell angles | 90.00, 89.78, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.30600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.173 | 0.970 |
| Total number of observations | 166764 * | |
| Number of reflections | 49658 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 8.5 | 293 | 20% Polyethylene glycol 8000, sodium-potassium phosphate, TRIS, imidazole, sodium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.8 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | imidazole-malate | 0.2 (M) | pH7.0 |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
