1FUP

FUMARASE WITH BOUND PYROMELLITIC ACID

Experimental procedure

Temperature [K]	294
Detector technology	AREA DETECTOR
Collection date	1995
Detector	SIEMENS
Spacegroup name	C 2 2 21
Unit cell lengths	104.000, 219.900, 86.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.185
Rwork	0.185
R-free	0.23400
RMSD bond length	0.006
RMSD bond angle	21.020 *
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.073 *
Number of reflections	44413 *
Completeness [%]	98.5 *
Redundancy	6.34 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	citrate	300 (mM)
2	1	reservoir	PEG4000	14 (%)