1FUK

CRYSTAL STRUCTURE OF THE CARBOXY TERMINAL DOMAIN OF YEAST EIF4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-20
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	34.700, 52.100, 82.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.750
R-factor	0.228
Rwork	0.221
R-free	0.25200
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.820
High resolution limit [Å]	1.750	1.750
Rmerge	0.029	0.150
Number of reflections	15120
<I/σ(I)>	20.6
Completeness [%]	96.5 *	90.2
Redundancy	2.74	1.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	298	PEG 550mme, bme, ZnSO4, MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	unknown *	6.5	298	PEG 550mme, bme, ZnSO4, MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	mPEG550	20 (%)
2	1	1		1 (mM)
3	1	1	MES	100 (mM)