1FU1
CRYSTAL STRUCTURE OF HUMAN XRCC4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-02-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.892, 74.793, 87.307 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.700 |
| R-factor | 0.246 |
| Rwork | 0.231 |
| R-free | 0.26300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.408 |
| Number of reflections | 31338 | |
| <I/σ(I)> | 21.03 | |
| Completeness [%] | 98.1 | 95.9 |
| Redundancy | 13.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 5 | 1 | reservoir | magnesium acetate | 10 (mM) |
