1FTR
FORMYLMETHANOFURAN:TETRAHYDROMETHANOPTERIN FORMYLTRANSFERASE FROM METHANOPYRUS KANDLERI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 157.500, 157.500, 242.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24800 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.488 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA) |
| Phasing software | MLPHARE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.870 |
| High resolution limit [Å] | 1.700 | 1.730 |
| Rmerge | 0.054 | 0.054 |
| Number of reflections | 135354 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 87.8 | 66 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | Shima, S., (1996) Proteins Struct.Funct.Genet., 26, 118. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MOPS | 50 (mM) | |
| 2 | 1 | drop | dithiothreitol | 2 (mM) | |
| 3 | 1 | drop | enzyme | 12-16 (mg/ml) | |
| 4 | 1 | reservoir | PEG8000 | 22 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.3 (M) | |
| 6 | 1 | reservoir | MOPS | 0.1 (M) |
