1FTK

CRYSTAL STRUCTURE OF THE GLUR2 LIGAND BINDING CORE (S1S2I) IN COMPLEX WITH KAINATE AT 1.6 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	110
Detector technology	CCD
Collection date	1998-06-20
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	43.360, 63.120, 46.380
Unit cell angles	90.00, 92.79, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.216 *
Rwork	0.216
R-free	0.26100
RMSD bond length	0.009
RMSD bond angle	1.441 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.030	0.590
Total number of observations	154641 *
Number of reflections	31151
<I/σ(I)>	18.3
Completeness [%]	94.4	70.4
Redundancy	4.96	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	4 *	15% PEG 8000 50 mM potassium phosphate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)
2	1	drop		20 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	kainate	10 (mM)
5	1	reservoir	PEG1450	8-15 (%)
6	1	reservoir		0.1 (M)