1FT6

REDUCED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1998-12-23
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	147.240, 147.240, 33.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.197 *
Rwork	0.197
R-free	0.23600
RMSD bond length	0.016
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.051	0.288
Total number of observations	112676 *
Number of reflections	24588
<I/σ(I)>	28.5
Completeness [%]	95.5	85.9
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.1	22 *	Iverson, T.M., (1998) Nat.Struct.Biol., 5, 1005. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	potassium phosphate	62.5 (%(w/v))	pH10.1
3	1	reservoir	potassium phosphate	92.5 (%(w/v))	pH10.1