1FT0

CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI K113A MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-06
Detector	MAR CCD 165 mm
Spacegroup name	H 3 2
Unit cell lengths	128.490, 128.490, 164.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
Rwork	0.226
R-free	0.26000
RMSD bond length	0.007
RMSD bond angle	26.810 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.058	0.330 *
Total number of observations	73487 *
Number of reflections	15110
<I/σ(I)>	16.8
Completeness [%]	92.8	94.9
Redundancy	4.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6	293	ammonium sulfate, Na/K tartrate, and sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-15 (mg/ml)
2	1	reservoir	ammonium sulfate	50 (%sat)
3	1	reservoir	sodium/potassium tartrate	200 (mM)
4	1	reservoir	sodium citrate	100 (mM)