1FSZ

CRYSTAL STRUCTURE OF THE CELL-DIVISION PROTEIN FTSZ AT 2.8A RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-09
Detector	MARRESEARCH
Spacegroup name	I 21 3
Unit cell lengths	159.140, 159.140, 159.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.199
Rwork	0.199
R-free	0.28200
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	30.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.800	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.065	0.310 *
Number of reflections	17024
<I/σ(I)>	9.9	2.9
Completeness [%]	99.0	99.7
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.62		pH 6.62

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG400	17 (%)
3	1	reservoir	MES	0.1 (M)
4	1	reservoir	ethanol	6 (%)