1FST
CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI TRIPLE MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-06 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | H 3 |
| Unit cell lengths | 125.210, 125.210, 82.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| Rwork | 0.213 |
| R-free | 0.26100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.048 | 0.267 |
| Total number of observations | 49713 * | |
| Number of reflections | 13241 | |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 293 | PEG 3400, isopropanol, HEPES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-15 (mg/ml) | |
| 2 | 1 | reservoir | PEG3400 | 12-14 (%) | |
| 3 | 1 | reservoir | 2-propanol | 5 (%) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) |
