1FST

CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI TRIPLE MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-06
Detector	MAR CCD 165 mm
Spacegroup name	H 3
Unit cell lengths	125.210, 125.210, 82.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.213
R-free	0.26100
RMSD bond length	0.007
RMSD bond angle	25.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.780
High resolution limit [Å]	2.700	2.700
Rmerge	0.048	0.267
Total number of observations	49713 *
Number of reflections	13241
<I/σ(I)>	17.9
Completeness [%]	99.6	99.6
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	293	PEG 3400, isopropanol, HEPES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-15 (mg/ml)
2	1	reservoir	PEG3400	12-14 (%)
3	1	reservoir	2-propanol	5 (%)
4	1	reservoir	HEPES	100 (mM)