1FSG
TOXOPLASMA GONDII HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE COMPLEXED WITH 9-DEAZAGUANINE, ALPHA-D-5-PHOSPHORIBOSYL-1-PYROPHOSPHATE (PRPP) AND TWO MG2+ IONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.330, 90.180, 75.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.050 |
| Rwork | 0.122 |
| R-free | 0.15430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED T. GONDII HGPRT-GMP COMPLEX STRUCTURE. LOOPS WATERS AND GMP WERE REMOVED FROM ALL SUBUNITS OF THE MOLECULAR REPLACEMENT MODEL. |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.340 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.080 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.072 | 0.555 |
| Total number of observations | 915734 * | |
| Number of reflections | 220433 * | |
| <I/σ(I)> | 8.4 | 1.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | drop was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | PRPP | 2 (mM) | |
| 4 | 1 | drop | 9-dzG | 2 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | precipitant |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | precipitant |
| 7 | 1 | reservoir | 200 (mM) | precipitant | |
| 8 | 1 | reservoir | beta-octylglucoside | 0.5 (%) |
