1FS6

GLUCOSAMINE-6-PHOSPHATE DEAMINASE FROM E.COLI, T CONFORMER, AT 2.2A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-01-05
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	126.800, 126.800, 139.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.214 *
Rwork	0.214
R-free	0.23500
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.067	0.316
Number of reflections	31742 *
<I/σ(I)>	9.64
Completeness [%]	94.5 *	70.8 *
Redundancy	3.7	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	291	HEPES, sodium acetate, pH 6.8, VAPOR DIFFUSION, HANGING DROP at 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	sodium acetate	2.45 (M)
3	1	reservoir	HEPES	100 (mM)	pH6.8