1FS0

COMPLEX OF GAMMA/EPSILON ATP SYNTHASE FROM E.COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9793, 0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	76.720, 176.090, 67.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.227
Rwork	0.216
R-free	0.26900
RMSD bond length	0.085
RMSD bond angle	1.650
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.020	2.020
Rmerge	0.053	0.256
Number of reflections	26978
<I/σ(I)>	20
Completeness [%]	89.3	63.2
Redundancy	6.1	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	22 *	0.5M tartrate, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.8	22 *	0.5M tartrate, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	tartrate	0.5 (M)
2	1	reservoir	Tris-HCl	0.1 (M)