1FRX

STRUCTURE AND PROPERTIES OF C20S FDI MUTANT

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	55.250, 55.250, 95.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.229
Rwork	0.229
RMSD bond length	0.017
RMSD bond angle	3.540
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *	2.500 *
Total number of observations	44495 *
Number of reflections	5700
Completeness [%]	91.9	96.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *	2 *	Shen, B., (1994) J.Biol.Chem., 269, 8564. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-10 (mg/ml)
2	1	drop	potassium phosphate	0.5 (M)
3	1	drop	ammonium sulfate	1.2 (M)
4	1	reservoir	ammonium sulfate	3.5 (M)