1FRQ

FERREDOXIN:NADP+ OXIDOREDUCTASE (FERREDOXIN REDUCTASE) MUTANT E312A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	C 1 2 1
Unit cell lengths	90.700, 57.900, 68.200
Unit cell angles	90.00, 99.50, 90.00

Refinement procedure

Resolution	10.000 - 1.950
R-factor	0.19
Rwork	0.190
Structure solution method	OTHER
Starting model (for MR)	1fnb
RMSD bond length	0.016
RMSD bond angle	25.200 *
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.105 *	0.287 *
Total number of observations	56640 *
Number of reflections	25030
<I/σ(I)>	8.1	2.3
Completeness [%]	97.8	96.4
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	4 *	drop is produced by a 1:1(v/v) mixture of the stored FNR stock with a reservoir *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.5 (M)
2	1	reservoir	sodium citrate/phosphate	0.2 (M)	McIlvaine's buffer