1FRN
THE INVOLVEMENT OF SER96 IN THE CATALYTIC MECHANISM OF FERREDOXIN-NADP+ REDUCTASE: STRUCTURE-FUNCTION RELATIONSHIP AS STUDIED BY SITE-DIRECTED MUTAGENESIS AND X-RAY CRYSTALLOGRAPHY
Experimental procedure
| Collection date | 1994-06-15 |
| Detector | SDMS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.700, 57.700, 68.100 |
| Unit cell angles | 90.00, 100.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.162 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.400 |
| Data reduction software | SDMS |
| Refinement software | TNT (5A) |
Data quality characteristics
| Overall | |
| Rmerge | 0.088 |
| Number of reflections | 22757 * |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium acetate | 0.1 (M) | |
| 2 | 1 | 1 | ammonium sulfate | 1.55-1.60 (M) | |
| 3 | 1 | 1 | protein | 3.4 (mg/ml) |
