1FRD

MOLECULAR STRUCTURE OF THE OXIDIZED, RECOMBINANT, HETEROCYST (2FE-2S) FERREDOXIN FROM ANABAENA 7120 DETERMINED TO 1.7 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 65
Unit cell lengths	44.200, 44.200, 80.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.167
RMSD bond length	0.009
RMSD bond angle	2.930
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.680 *
Completeness [%]	99.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		referred to 'Jacobson, B.L.', (1992) Arch. Biochem. Biophys., 294, 279-281 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	bis-Tris propane	50 (mM)	pH7.0
3	1	reservoir	ammonium sulfate		pH4.5-9.0
4	1	reservoir	PEG8000