1FR8
CRYSTAL STRUCTURE OF THE BOVINE BETA 1,4 GALACTOSYLTRANSFERASE (B4GALT1) CATALYTIC DOMAIN COMPLEXED WITH URIDINE DIPHOSPHOGALACTOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-17 |
| Detector | CUSTOM-MADE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 104.720, 161.370, 106.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.26200 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.065 | 0.425 |
| Total number of observations | 138168 * | |
| Number of reflections | 32664 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 91.9 | 94 * |
| Redundancy | 4.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 20 * | PEG 5000, Tris, MnCl2, Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-15 (mg/ml) | |
| 2 | 1 | drop | Tris Maleate | 20 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | reservoir | PEG5000 | 25-30 (%) | |
| 6 | 1 | reservoir | 100-150 (mM) | ||
| 7 | 1 | reservoir | Tris | 100 (mM) |
