1FR6

REFINED CRYSTAL STRUCTURE OF BETA-LACTAMASE FROM CITROBACTER FREUNDII INDICATES A MECHANISM FOR BETA-LACTAM HYDROLYSIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	1989-01-01
Detector	XENTRONICS
Spacegroup name	P 21 21 21
Unit cell lengths	98.070, 84.630, 89.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.186
RMSD bond length	0.027
RMSD bond angle	2.800
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	CCP4
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Rmerge	0.090
Number of reflections	22927
Completeness [%]	84.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	1.6M sodium-potassium phosphate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K