1FQW
CRYSTAL STRUCTURE OF ACTIVATED CHEY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.684, 53.911, 161.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.370 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24000 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.410 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.075 | 0.230 |
| Total number of observations | 89177 * | |
| Number of reflections | 17703 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 89.1 | 52.2 |
| Redundancy | 5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 * | 298 | Ammonium sulfate, Tris buffer, MnCl2, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mM) | |
| 2 | 1 | drop | 8 (mM) | ||
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 4 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 6 | 1 | reservoir | glycerol | 5-10 (%) | |
| 7 | 1 | reservoir | Tris | 100 (mM) |
