1FQ1
CRYSTAL STRUCTURE OF KINASE ASSOCIATED PHOSPHATASE (KAP) IN COMPLEX WITH PHOSPHO-CDK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-01 |
| Detector | ADSC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 134.450, 134.450, 65.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.235 * |
| Rwork | 0.235 |
| R-free | 0.31300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.073 | 0.360 |
| Total number of observations | 89741 * | |
| Number of reflections | 17091 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 99.7 | 99.6 * |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 8000,KCl, Mgacetate,Na cacodylate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | AMPPNP | 5 (mg) | |
| 3 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 8-10 (%(w/v)) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | magnesium acetate | 100 (mM) |
