1FPS

CRYSTAL STRUCTURE OF RECOMBINANT FARNESYL DIPHOSPHATE SYNTHASE AT 2.6 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	I 41 2 2
Unit cell lengths	88.600, 88.600, 276.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.194
Rwork	0.194
RMSD bond length	0.012
RMSD bond angle	1.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.160 *
Rmerge	0.115 *
Number of reflections	25159 *
Completeness [%]	80.1 *
Redundancy	2.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	reservoir	tartrate	0.78 (M)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	phosphate	20 (mM)