1FPO

HSC20 (HSCB), A J-TYPE CO-CHAPERONE FROM E. COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1999-03-01
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	123.010, 69.990, 68.110
Unit cell angles	90.00, 98.08, 90.00

Refinement procedure

Resolution	50.000 * - 1.800
R-factor	0.216
Rwork	0.216
R-free	0.24600
RMSD bond length	0.005
RMSD bond angle	0.912 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.045
Total number of observations	171482 *
Number of reflections	53718 *
<I/σ(I)>	12.7
Completeness [%]	98.7 *	97.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	295	PEG400 Hepes Magnesium Chloride Dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	295	PEG400 Hepes Magnesium Chloride Dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	20 (%(w/v))
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir		0.1 (M)
4	1	reservoir	dioxane	1-10 (%(w/v))