1FPN

HUMAN RHINOVIRUS SEROTYPE 2 (HRV2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-07-15
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	308.680, 352.980, 380.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.18
RMSD bond length	0.012
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.108	0.230
Number of reflections	392077
<I/σ(I)>	7.5
Completeness [%]	58.8	55.8
Redundancy	1.3	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	293	ammonium sulphate, potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	293	ammonium sulphate, potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus	5 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	ammonium sulfate	0.4 (M)
4	1	reservoir	sodium/potassium phosphate	0.1 (M)