1FPE

STRUCTURAL ASPECTS OF THE ALLOSTERIC INHIBITION OF FRUCTOSE-1,6-BISPHOSPHATASE BY AMP: THE BINDING OF BOTH THE SUBSTRATE ANALOGUE 2,5-ANHYDRO-D-GLUCITOL-1,6-BISPHOSPHATE AND CATALYTIC METAL IONS MONITORED BY X-RAY CRYSTALLOGRAPHY

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	61.000, 166.500, 79.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.18
Rwork	0.180
RMSD bond length	0.018
RMSD bond angle	1.830
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.069 *
Total number of observations	87818 *
Number of reflections	36591
Completeness [%]	81.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4		Ke, H., (1989) J. Mol. Biol., 212, 513. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	5-10 (mg/ml)
2	1	2	Tris base	20 (mM)
3	1	2	maleic acid	2.5 (mM)
4	1	2	EDTA	0.1 (mM)
5	1	2		0.3 (mM)
6	1	2	PEG3350	4 (%(w/v))