1FPB

CRYSTAL STRUCTURE OF THE NEUTRAL FORM OF FRUCTOSE 1,6-BISPHOSPHATASE COMPLEXED WITH REGULATORY INHIBITOR FRUCTOSE 2,6-BISPHOSPHATE AT 2.6-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	131.400, 131.400, 68.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.600
R-factor	0.174
Rwork	0.174
RMSD bond length	0.171
RMSD bond angle	2.880
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.070 *
Total number of observations	92333 *
Number of reflections	19145 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10-15 (mg/ml)
2	1	2	Tris	20 (mM)
3	1	2	maleic acid	2 (mM)
4	1	2	EDTA	0.1 (mM)
5	1	2		5 (mM)
6	1	2	2-mercaptoethanol	1 (mM)
7	1	2	PEG3350	7.0 (%(w/v))
8	1	2	Fru-6-P	1 (mM)