1FP3
CRYSTAL STRUCTURE OF N-ACYL-D-GLUCOSAMINE 2-EPIMERASE FROM PORCINE KIDNEY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-04-19 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.138, 97.193, 100.651 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.970 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.24400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 * |
| Data scaling software | SADIE |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.980 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.056 | 0.056 |
| Total number of observations | 124509 * | |
| Number of reflections | 39143 | |
| Completeness [%] | 82.1 | 82.1 |
| Redundancy | 2.5 | 2.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | Maru, I., (1996) J. Biochem., 120, 481. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | pH7.2 |
| 2 | 1 | drop | protein | 5 (mg/ml) | |
| 3 | 1 | drop | PEG6000 | 5.5 (%(w/v)) | |
| 4 | 1 | drop | ammonium acetate | 100 (mM) | |
| 5 | 1 | drop | sodium citrate | 50 (mM) | pH5.0 |
| 6 | 1 | reservoir | PEG6000 | 11 (%(w/v)) | |
| 7 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 8 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.0 |
