1FOH
PHENOL HYDROXYLASE FROM TRICHOSPORON CUTANEUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.030, 150.680, 114.920 |
| Unit cell angles | 90.00, 114.68, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.400 |
| R-factor | 0.217 * |
| Rwork | 0.217 |
| R-free | 0.27800 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.095 * | 0.335 * |
| Number of reflections | 118897 | |
| <I/σ(I)> | 7.8 | 2 |
| Completeness [%] | 98.1 | 94.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 20 (mg/ml) | |
| 2 | 1 | drop | phenol | 20 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 120 (mM) | or 0.21M KCl and 16% PEG4000 |
| 4 | 1 | reservoir | ammonium acetate | 0.52 (M) |
