1FOC
Cytochrome C557: improperly folded thermus thermophilus C552
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.720, 69.050, 36.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| Rwork | 0.273 |
| R-free | 0.42320 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.058 | 0.526 |
| Number of reflections | 5309 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.5 | 89.8 |
| Redundancy | 6.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 42-46% MPEG 2K (w/v), 0.1M Na cacodylate pH 7.0, 50mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 24K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 42-46% MPEG 2K (w/v), 0.1M Na cacodylate pH 7.0, 50mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 24K |
