1FOB

CRYSTAL STRUCTURE OF BETA-1,4-GALACTANASE FROM ASPERGILLUS ACULEATUS AT 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2000-01-27
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	59.700, 87.980, 128.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.590 - 1.800
R-factor	0.211
Rwork	0.211
R-free	0.25200 *
RMSD bond length	0.006
RMSD bond angle	22.600 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.590	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.041	0.354
Number of reflections	31237
<I/σ(I)>	14.5
Completeness [%]	98.7	97.7 *
Redundancy	4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Ryttersgaard, C., (1999) Acta Crystallogr.,Sect.D, 55, 929. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	30 (%(v/v))
2	1	reservoir		0.2 (M)
3	1	reservoir	sodium HEPES	0.1 (M)	pH7.5