1FO9
CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINYLTRANSFERASE I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-04-24 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.478, 82.423, 102.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.240 - 1.500 |
R-factor | 0.166 |
Rwork | 0.166 |
R-free | 0.19400 |
RMSD bond length | 0.013 |
RMSD bond angle | 24.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.240 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.065 | 0.272 |
Total number of observations | 401605 * | |
Number of reflections | 99934 | |
<I/σ(I)> | 10.8 | |
Completeness [%] | 94.2 | 77.8 |
Redundancy | 4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 * | 295 | PEG 8000, potassium chloride, glycerol, Tris, MES, UDP-GlcNAc, manganese chloride, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MES | 10 (mM) | |
3 | 1 | drop | 270 (mM) | ||
4 | 1 | drop | 2 (mM) | ||
5 | 1 | drop | UDP-GlcNAc | 10 (mM) | |
6 | 1 | reservoir | PEG8000 | 15-25 (%) | |
7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
8 | 1 | reservoir | glycerol | 0-5 (%) |