1FO1

CRYSTAL STRUCTURE OF THE RNA-BINDING DOMAIN OF THE MRNA EXPORT FACTOR TAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-10-14
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	96.354, 96.354, 152.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.25
Rwork	0.250
R-free	0.27800
RMSD bond length	0.007
RMSD bond angle	1.260
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.057	0.235
Total number of observations	67191 *
Number of reflections	16300
<I/σ(I)>	21
Completeness [%]	98.5	93
Redundancy	4.1 *	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	4 *	PEG 8000, EDTA, cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)
3	1	reservoir	PEG8000	18 (%(w/v))
4	1	reservoir	EDTA	20 (mM)