1FO0
MURINE ALLOREACTIVE SCFV TCR-PEPTIDE-MHC CLASS I MOLECULE COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1999-12-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 76.580, 120.420, 102.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.500 |
R-factor | 0.218 |
Rwork | 0.225 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1KB5 1vac |
RMSD bond length | 0.011 |
RMSD bond angle | 0.030 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (4.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.100 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.081 * | 0.375 * |
Number of reflections | 33614 | |
<I/σ(I)> | 8.3 | 1.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.2 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 4 * | PEG 6000 10% HEPES 0.1M PH 7.0 MGAC 0.25 M NACL 0.25M, pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 10 (%) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | |
4 | 1 | reservoir | magnesium acetate | 0.25 (M) |