1FNZ

A bark lectin from robinia pseudoacacia in complex with N-acetylgalactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-05-22
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	64.622, 76.231, 118.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.050
R-factor	0.197 *
Rwork	0.197
R-free	0.22200
RMSD bond length	0.006
RMSD bond angle	1.392
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.086	0.278
Total number of observations	53508 *
Number of reflections	18204
<I/σ(I)>	11.87
Completeness [%]	77.2	96.5
Redundancy	2.94	2.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	277	Rabijns, A., (2000) Acta Crystallogr., Sect.D, 56, 1638. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	100 (mg/ml)
2	1	drop	Tris	0.1 (M)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	PEG4000	30 (%(w/v))