1FNY

LEGUME LECTIN OF THE BARK OF ROBINIA PSEUDOACACIA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-18
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	64.078, 75.854, 118.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.810
R-factor	0.189 *
Rwork	0.189
R-free	0.21100
RMSD bond length	0.005
RMSD bond angle	1.422
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.049	0.258
Total number of observations	97833 *
Number of reflections	22296
<I/σ(I)>	22.45
Completeness [%]	87.4	100
Redundancy	3.67	3.56

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	277	Rabijns, A., (2000) Acta Crystallogr., Sect.D, 56, 1638. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	100 (mg/ml)
2	1	drop	Tris	0.1 (M)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	PEG4000	30 (%(w/v))