1FNL

CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF A HUMAN FCGRIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 2
Unit cell lengths	67.427, 85.733, 36.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.184 *
Rwork	0.184
R-free	0.21600
RMSD bond length	0.013 *
RMSD bond angle	1.730 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.054	0.210
Number of reflections	36180 *
<I/σ(I)>	29.5
Completeness [%]	95.8	80.1
Redundancy	3.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	298	PEG 8000, Sodium Hepes, pH 6.5, EVAPORATION, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%)
2	1	reservoir	sodium HEPES	50 (mM)
3	1	drop	Tris	50 (mM)
4	1	drop		50 (mM)
5	1	drop	protain	0.010 (mM)